ABSTRACT
Background and Objective: Probiotc bacteria have benefical effect on consumer health. This study was done to investigate the antimicrobial effect of several probiotic in combinations with different prebiotics against food patoghenic bacteria including Staphylococcus aureus and Listeria monocytogenes
Methods: In this descriptive - analytical study, probiotics including Lactobacillus plantarum, L. acidophilus, L. fermntum, L. casei and L. rhamnosus with prebiotics [1%] including raffinose, lactulose, inulin and trehalose were cultured in MRS broth for 24 hours at 30[degree]C in anaerobic conditions. Antimicrobial property of them was determined with well diffusion plate's method
Results: Probiotics in the presence of prebiotics indicated the higher antimicrobial effect compared to probiotics alone [P<0.05]. The application of prebiotics such as L. casei with raffinose showed higher antimicrobial property against Listeria monocytogenes than the free prebiotics consumption. The diameter of inhibitory growth zone in the presence of raffinose as a prebiotics was 14.66 mm and its absence reduced to 11.75 mm
Conclusion: Antimicrobial effect of probiotics in combination with prebiotics against Staphylococcus aureus and Listeria monocytogenes was higher than probiotics consumption alone